Analysis of Levodopa Content in Commercial Mucuna pruriens Products Using High-Performance Liquid Chromatography with Fluorescence Detection

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P01.36. Assessment of commercial formulations of mucuna pruriens seeds for Levodopa (L-DOPA) content

Purpose Mucuna pruriens (mucuna) seeds contain 3-6% LDOPA, and have been used in traditional Ayurvedic medicine to treat diseases resembling Parkinson’s disease (PD). Pilot studies in PD show that mucuna seed powder has similar effects to conventional levodopa/carbidopa medication. Formulations of mucuna seed are readily available through the internet, and are used by some PD patients as an alt...

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Levodopa in Mucuna pruriens and its degradation

Mucuna pruriens is the best known natural source of L-dopa, the gold standard for treatment of Parkinsonism. M. pruriens varieties are protein rich supplements, and are used as food and fodder worldwide. Here, we report L-dopa contents in seeds of fifty six accessions of four M. pruriens varieties, M. pruriens var. pruriens, M. pruriens var. hirsuta, M. pruriens var. utilis and M. pruriens var....

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Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection

Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of 20 trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile 4.5:85:5:45.5, v/v/v/v. Results: Desp...

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High performance liquid chromatography analysis of MESNA (2-mercaptoethane sulfonate) in biological samples using fluorescence detection.

MESNA is the sodium salt of 2-mercaptoethane sulfonate, a thiol-containing drug. It is an antioxidant used particularly in renal protection. Several studies have proved that MESNA has beneficial effects in ischemic acute renal failure where it scavenges reactive oxygen species (ROS), due to the presence of the thiol group. It also reduces the size of urinary bladder cancer. MESNA was proved to ...

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Simultaneous determination of furosemide and amiloride in plasma using high-performance liquid chromatography with fluorescence detection.

A high-performance liquid chromatographic method using fluorescence detection for the simultaneous determination of furosemide and amiloride is described. The chromatographic system is based on reversed-phase ion-pair chromatography with sodium dodecylsulphate as ion-pairing agent. The same counter-ion is used for the ion-pair liquid-liquid extraction to ethyl acetate. The minimum detectable co...

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ژورنال

عنوان ژورنال: The Journal of Alternative and Complementary Medicine

سال: 2018

ISSN: 1075-5535,1557-7708

DOI: 10.1089/acm.2017.0054